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KMID : 1098420050130040199
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Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 4 p.199 ~ p.205
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Genotoxicity and Cytotoxicity in Human Cancer and Normal Cell Lines of the Extracts of Rhododendron brachycarpum D. Don leaves
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Byun Kyoung-Sup
Lee Young-Woo
Jin Hyou-Ju
Lee Mi-Kyoung
Lee Hyeon-Yong
Lee Kun-Jae
Heo Moon-Young
Yu Chang-Yeon
Lee Jin-Ha
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Abstract
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This study was carried out to investigate the effect of 70% ethanol extract and each fraction from Rhododendron brachycarpum D. Don leaves on cytotoxicity, anticancer, genotoxicity and immunological activity in vitro bioassay. Cytotoxicity for human normal cells (HEL299 and Chang) of the samples was shown below 35% in 0.5 mg/ml concentration of samples except aqueous fraction by SRB assay. DNA damage on the Chang cell of the samples alone in comet assay was observed very weak damage activity even in high concentration (1 mg/ml) of the samples. The anticancer effect of the samples on human cancer cell lines (A549, AGS, Hep3B, MCF7) was indicated that the cancer cells were inhibited gradually in proportion to the increase of the concentration of the samples by MTT assay. The growth of the Raji and Jurkat cells were hastened by adding butanol fraction among the samples. In the genotoxicity on H_2O_2-induced DNA damage in Chang cells using alkaline comet assay, most of samples were shown a strong protective activity from DNA OTM values.
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KEYWORD
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Rhododendron brachycarpum D. Don, genotoxicity, cytotoxicity, anticancer, DNA, Comet, MTT, SRB
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